CUSTOM ITEM: HPLC Column, Bidentate C8 300, 5um, 2.1mm ID x 50mm Length, 300A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40008‑05P‑2‑3M Cogent Bidentate C8™ 300 HPLC Column is a custom‑manufactured, wide‑pore reversed‑phase column designed specifically for the analysis of large molecules, including proteins, peptides, polysaccharides, and other macromolecular analytes. Built on Cogent TYPE‑C™ silica hydride technology, this column delivers excellent selectivity, faster equilibration, and extremely robust column lifetime compared with conventional 300 Å silica‑based C8 columns.
The stationary phase uses an aliphatic C8 hydrocarbon chain bonded via direct silicon–carbon bonds to a silica hydride surface. This chemistry produces a phase that is more hydrophobic than traditional C8 columns, yet more forgiving than C18 for large biomolecules. The 300 Å pore structure enables efficient analyte access for macromolecules while maintaining the distinctive selectivity benefits of TYPE‑C™ hydride surfaces.
Unlike typical wide‑pore C8 materials, the Bidentate C8™ 300 can retain compounds that often show poor or inconsistent retention on standard silica phases. The column is also stable at very low pH (below pH 2) without degradation and can retain select hydrophilic macromolecules, especially when operated in HILIC‑like modes—a unique capability among wide‑pore C8 columns.
The 2.1 mm internal diameter and 50 mm length provide short run times, reduced solvent consumption, and improved detector sensitivity. This format is widely favored for LC‑MS of large biomolecules, provided the system is capable of precise low‑flow delivery and uses reduced‑volume detector flow cells.
Why Choose Bidentate C8™ 300 for Macromolecules
This column is typically selected when:
Analytes are large biomolecules (proteins, peptides, polysaccharides)
Standard 100–120 Å pores restrict macromolecular access
Conventional wide‑pore C8 lacks sufficient retention or stability
Low‑pH operation is required without column damage
LC‑MS sensitivity and short run times are priorities
The combination of 300 Å pores and TYPE‑C™ silica hydride chemistry provides a method window that conventional wide‑pore silica columns cannot match.
Key Features
Cogent TYPE‑C™ Silica Hydride Technology
Direct Si–C bonding for exceptional chemical robustness
Reduced silanol activity and secondary interactions
Faster equilibration than conventional silica‑based wide‑pore columns
Bidentate C8™ 300 Bonding
Enhanced hydrophobicity vs standard C8
Suitable balance for large biomolecules
Retains hydrophobic macromolecules without excessive adsorption
Wide‑Pore (300 Å) Architecture
Optimized for large molecular weight analytes
Improves mass transfer and peak shape for biomolecules
Effective for proteins and polysaccharides
LC‑MS‑Friendly Geometry
2.1 mm ID supports low‑flow operation and sensitivity
50 mm length enables fast analysis
Requires precise low‑flow pumping and reduced‑volume flow cells
Aggressive Method Compatibility
Stable at very low pH (below 2)
Compatible with aqueous‑rich mobile phases
Supports HILIC‑like operation for select macromolecules
System Requirements
For optimal performance with this narrow‑bore wide‑pore column, use:
A pump capable of accurate, stable low‑flow delivery
Detector flow cells designed for low internal volume
LC‑MS systems configured for biomolecular analysis
Typical Applications
LC‑MS of proteins, peptides, and polysaccharides
Biomolecule characterization
Macromolecular purity and profiling
Wide‑pore reversed‑phase separations
Low‑pH biomolecule methods
Alternative HILIC‑mode macromolecule analysis
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable